View credits, reviews, tracks and shop for the Vinyl release of "Live And Direct" on Discogs. Discover releases, reviews, credits, songs, and more about Facs - Live And Direct at Discogs. Complete your Facs collection. Find out how to stain cells for flow cytometry using a conjugated primary antibody, with Abcam's direct flow cytometry protocol. Direct flow cytometry Fluorescence activated cell sorting (FACS) of live cells separates a population of This is the basic task of flow cytometry. Harvest, wash the cells (single cell suspension) and adjust cell number to a concentration of x cells/ml in ice cold FACS Buffer (PBS, % BSA or. Select FSC-A versus ZY+Lineage (appear in same channel; See Note at step 9 on antibody choice) to identify live Lineage- cells that are ZY- . Find microsphere products for flow cytometry–based cell counting for a wide range of Invitrogen LIVE/DEAD BacLight Bacterial Viability and Counting Kit. Only live cells with metabolic activity generate enough ROS to oxidize H2DCFDA, which makes it a robust reporter for cell viability as well as. flow cytometry within reach for new and experienced researchers alike. combinations you can analyze and automation for direct counting of cells. Direct Immunofluorescence Staining of Surface Epitopes of Cells and Blood. in live cells without requiring permeabilization of the cell membrane.